Prime 95B™ > Photometrics

Scientific CMOS Camera, 95% Quantum Efficiency

 

Outperforming EMCCD cameras, the Prime 95B Scientific CMOS (sCMOS) camera series includes several back illuminated sCMOS cameras that offer 95% QE with extreme sensitivity and high frame rates.

* 95% Quantum Efficiency

* 11 µm x 11 µm Pixel Area 

* 3 Field of View Options (18mm, 22mm, 25mm)

Key Features

Sensitivity 

The Prime 95B back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency.

By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. Combining this with large, 11 µm pixels results in a camera technology as sensitive as EMCCD with greater stability, faster speeds and no gain aging. 

 

Prime 95B is the Scientific CMOS with extreme sensitivity using high Quantum Efficiency (QE) Backside Illumination (BSI), a first for Scientific CMOS cameras. The 95B’s sensor converts up to 95% of incident photons into a measurable signal. Unlike microlens approaches to increasing QE, which lose effectiveness as objective magnification is increased, Prime 95B’s BSI sensor brings light into the pixel photodiode from behind, avoiding structures that reflect or absorb light. When combined with large 11μm pixels, Prime 95B can deliver over 300% more signal than other sCMOS cameras at 100X magnification.
More importantly, Prime 95B outperforms EMCCD cameras?with no excess noise that negates the benefit of using a high QE sensor, and additional limitations from EM gain calibration, stability, expense, and sensor lifetime With a true 16-bit dynamic range, Prime 95B easily accomplishes what EMCCD can not?detect weak and bright signals within the same image with photon-noise limited performance.
The extreme sensitivity not only allows fainter signals to be detected, it provides the flexibility to increase frame rates, or turn down the excitation intensity to reduce cellular photo-damage. Yet Prime 95B maintains the same high frame rates, field-of-view and extremely low read noise that has made sCMOS so popular for live-cell imaging.

 

Specifications	Camera Performance
Sensor	GPixel GSense 144 BSI CMOS Gen IV, Grade 1 in imaging area
Active Array Size	1200 x 1200 pixels (1.44 Megapixel)
Pixel Area	11μm x 11μm (121μm2)
Sensor Area	13.2mm x 13.2mm  18.7mm diagonal
Peak QE%	>95%
Read Noise	1.3e- (Median)  1.5e- (rms)
Full-Well Capacity	80,000e- (Combined Gain)  4,500e- (High Gain)
Dynamic Range	61,500:1 (Combined Gain)
Bit Depth	16-bit (Combined Gain)  12-bit (High Gain)
Readout Mode	Rolling Shutter  Effective Global Shutter
Binning	2x2 (on FPGA)

 

Cooling Performance	Sensor Temperature	Dark Current
Air Cooled	-20ºC @ 30ºC Ambient	0.55 e-/pixel/second
Liquid Cooled	-25ºC @ 30ºC Ambient	0.3 e-/pixel/second

 

Specifications	Camera Interface
Digital Interface	PCIe, USB 3.0
Lens Interface	C-Mount
Mounting Points	2 x ¼ 20” mounting points per side to prevent rotation
Liquid Cooling	Quick Disconnect Ports

 

Triggering Mode	Function
Input Trigger Modes	Trigger-First - Sequence triggered on first rising edge  Edge - Each frame triggered on rising edge  SMART Streaming - Fast iteration through multiple exposure times
Output Trigger Modes	First Row - Expose signal is high while first row is acquiring data  Any Row - Expose signal is high while any row is acquiring data  All Rows - Effective Global Shutter ? Expose signal is high when all rows are acquiring data
Output Trigger Signals	Expose Out (up to four signals), Read Out, Shutter Out, Trigger Ready

 

Frame Rate (PCIe interface)
Array Size	16-bit	12-bit
1200 x 1200	40	80
1200 x 512	94	188
1200 x 256	188	374
1200 x 128	374	737

 

 

 

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