The InVi SPIM is the first commercially available inverted light-sheet microscope.
It is perfectly suited for a great variety of samples and applications raging from cell culture to whole embryos (e.g. Drosophila and Zebrafish)
- Inverted microscope optimized to image your most delicate biological samples
- 2 lens geometry (1x detection, 1x illumination)
- 1x Nikon 25x/1.1 NA water immersion/dipping
- 2x Hamamatsu Orca Flash 4.0 v3 (sCMOS)
- Up to 6 laser lines (diode, DPSS) from 405 to 685 nm
- Full environmental control: temperature, CO2, O2, humidity (SMC chiller/heater, ibidi gas mixer)
- Sample accessible from above, no rotation
- Easy to keep sample sterile
Illumination and Detection
The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling subcellular resolution in living samples free of phototoxic effects.
A Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens projects the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens.
An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.
- Chromatic correction from 440 to 660 nm
- Light-sheet generation by beam scanning
- Flexible light-sheet thickness (2 µm to 6 µm)
- Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens
- Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
- 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
- Filters adapted to the selected laser lines
- 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
- Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- Peak quantum efficiency (QE): 82% @ 560 nm
Sample Holder
- Trough of FEP foil allows separation of immersion water and sample medium
- Sample is dropped into trough and held by gravitation
- No agarose, glue, … required
- Partnered with ibidi to create a disposable chamber
Precise and Stable Environmental Control
Temperature accuracy
- Immersion medium
- Chamber lid
- Range: 20 - 37°C
Gas-concentration management
- CO2 and O2 dosage unit
- Range: 0 – 15% (CO2) / 1 – 21% (O2)
Humidity control
- Gas humidifier with feedback control
- Range: 20 – 99%
