The PMP102 is a state of the art microprocessor controlled pipette puller. The PMP102 is designed to pull a
pipette horizontally to produce two identical pulled micropipettes. Different kinds of pipettes can be pulled repeatedly
by the preset program sequences. There are varied micropipettes can be produced such as patch clamp electrodes
intracellular electrodes, injection micropipette and micro-needles by manufactory preset sequences.
To achieve these versatility and the higher degree of reproducibility, many advanced technologies are applied
on the PMP102:
Instead of mechanical tip length setting from other pipette puller, there is an exclusive optical-digital
ruler in the PMP102 to perform precise taper length setting, real-time measurement and controlling.
With this feature, a user can handle taper pulling very precisely and easily. Equipped with a
powerful computerize tip sensing function, the puller can automatically finish the tip pulling.
It gives the user a quick reference to pull an ideal tip.
Inside the PMP102, there is an advanced microcontroller to perform real-time heater monitoring
and controlling. If a heating level is selected and preset, the microcontroller will measure the
actual heating power during heating power on. The measurement is real-time displayed and
feedback to the control unit to match the set point dynamically. As a result, the puller always
provides precise heating power, despite of many times of pipette pulling or
thermal/electrical characteristic changing. If the heating level is set to AUTO, the heater will
automatically search the melting point for different glass pipettes. Under a microcomputer controlling,
the heater is smart and reliable.
There are 25 manufacture/user programmable and savable pulling sequences with 18 steps in
each sequence. Users can easily program different pipette tip size, tip length and tip shape in
different sequence for different application. Time and length count, heat level, heat control and
action parameters can be individually set in each step. After a special sequence setting up,
a double identical micropipettes will be automatically produced by just pressing the START
button on the PMP102.
apply precise controlled pneumatic pressure as the pulling force, which gives more controllable,
even and consistent dragging characteristics. With a double horizontal pulling, the PMP102 can
pull two identical ideal injection tips or microelectrode tips at the same time. Within a very compact
size, the PMP102 can precisely and automatically perform multistep pulling without manual interrupt.
A precision micro-linear ball bearing rail and advanced pneumatic components are used to provide no fault pulling movement. A simple 4x4 keypad and full information display LCD let users control easily
and read all pulling parameters directly, which include sequences, steps, time, timing, heater
level, heater control, tip length and actions. The heater power control and acting time count up or
count down are real-time displayed. With versatility of the intelligent PMP102, pulling an ideal
micropipette is no longer an uncertainty of hand skill, but a reproducible automatic processing.
The PMP102 pipette puller is well tested and pre-installed with a number of different pipette
pulling programs. A user can easily find out a right sample picture or the closest characteristic
from the sequence list on the User’s Manual. Select the corresponding sequence and just
pressing the START key, a desired pipette will be pulled automatically. The preset programs are convenient and important not only because they can pull many different pipettes, but also they
can be used as templets to produce new programs. With the copy function and just a few
modifications from an exist sequence, a new customize sequence program can be configured to
fit a special requirement. As many as 22 sequences are developed and preset to the PMP102 by
spending a lot of time and expertise.
These preset sequences are so important that users will save their time and pipettes
Since a good microforge also needs precise heater controlled power and air jet, the PMP102 just
has perfect features to satisfy this requirement. There is an optional output connector on the
PMP102 rear panel which supplies computerized heater power. A user can easily set heat grade
for a microforge heater as well as for the puller heater. The connector also supplies power for
microforge illumination light. There areoptional pressure output connector and foot switch connectors
in the front panel, which can output adjustable pressure air to an optional microforge for holding
pipette tip polishing, shaping or heater cooling. Both microforge heater and air jet can be controlled
by foot switches as well as key pad on the PMP102 front panel. With the precise heater power supply, light power supply and adjustable air pressure supply from the PMP102, a combinational microforge
can be built with less cost, more portable but more features.
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